As part of the Signal-Seeker™ product line, IgG control beads (CIG01) have been developed as highly specific negative control beads for Cytoskeleton"s mouse, monoclonal antibody-based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition. The IgG antibodies are covalently attached to minimize antibody heavy and light chain leaching, which makes interpretation of results easier. Additionally, these control beads are effective for pre-clearing lysates without risk of contamination with IgG antibody. IgG control beads (CIG01) are included in our comprehensive Signal-Seeker™ antibody based Detection Kits and utilizing these kits are recommend for first time PTM investigators.
Each lot of affinity-bead is quality controlled to provide high batch to batch consistency, see COA documents.
Application 1: Utilization of IgG control beads
IgG control beads are compatible with both the phosphotryosine and SUMOylation 2/3 affinity beads and Signal-Seeker kits. Note these beads do not have cross reactivity with either of these PTMs (Figure 1).
Figure 1: A431 cell were lysed with BlastR buffer. 1mg of protein was incubated with APY03-beads (pY) or CIG01 (IgG control). Samples were separated by SDS-PAGE and transferred to PVDF. Western blot using a phosphotyrosine antibody (APY03) was performed. 1mg of protein was incubated with ASM24-beads (SUM0 2/3) or CIG01 (IgG control).Samples were separated by SDS-PAGE and transferred to PVDF. Western blot using a SUMO 2/3 antibody (ASM23) was performed.
Amount:Each package contains enough IgG control beads for 10 reactions.
For more information contact: signalseeker@cytoskeleton.com
Associated Products:
Signal-Seeker™ Phosphotyrosine Detection Kit (Cat. # BK160)
Signal-Seeker™ SUMOylation 2/3 Detection Kit (Cat. # BK162)
Signal-Seeker™ Phosphotyrosine Affinity Beads (Cat.# APY03-beads)
Signal-Seeker™ SUMOylation 2/3 Affinity Beads (Cat.# ASM24-beads)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
Click on the pdf icon below to download the datasheet 
For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here
Visit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。
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