SiR700-lysosome is based on the pepstatin A (a cathepsin D binding peptide) which has been labeled with the proprietary far-red fluorophore called silicon rhodamine 700 (SiR700). SiR700-lysosome is fluorogenic, cell permeable and highly specific for lysosomes. It stains early and late endocytic vesicles and lysosomes in live cells without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-lysosome is compatible in dual fluorescence studies with other SIR dye conjugates, GFP and/or mCherry fluorescent proteins. It can be imaged with standard Cy5 filter sets, or high resolution filters for dual staining. SiR700-lysosome can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 35 – 140 staining experiments*.
Optical properties
λabs 689 nmλex 716 nm
εmax 1.0·105 mol-1·cm-1
MW 1262.7 g/mol
MF C69H103N9O11Si
*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 µM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.
Cytoskeleton, Inc. is the exclusive provider of Spirochrome, Ltd. products in North America.
Live human fibroblast cells stained with 1 µM SiR700-lysosome (red) and Hoechst (blue) for 1 h at 37°C and imaged by widefield microscopy.
For product Datasheets and MSDSs please click on the PDF links below.
Originating Publication
Lukinavicius G. et al. 2016. Fluorogenic probes for multi-color imaging in living cells. J. Am. Chem. Soc. Doi: 10.1021/jacs.6b04782.
Lukinavicius, G. et al. 2015. SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy. Nat. Commun. 6:8497 doi:10.1038/ncomms9497 (2015).
For comparison information with other nuclear stains click here.
Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。
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