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主营:微管蛋白、肌动蛋白、小G蛋白、GAPs、GEFs
℡ 4000-520-616
℡ 4000-520-616
Cytoskeleton/SUMO-2/3 Antibody Mouse Monoclonal (Clone 11G2)/ASM24-S
产品编号:ASM24-S
市  场 价:¥2500.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>抗大鼠>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$125.00
品      牌: Cytoskeleton
公      司:Cytoskeleton
公司分类:
Cytoskeleton/SUMO-2/3 Antibody Mouse Monoclonal (Clone 11G2)/ASM24-S
商品介绍

Host/IsotypeMouse/IgG1-kappa

Clone11G2

SpeciesReactivityBroadReactivity

ValidationDataWhitePaperAntibodypedia

Anti-SUMO-2/3antibodyisamousemonoclonalantibody.Theantibodywasraisedagainstfull-lengthrecombinantSUMO-2protein(Uniprot:P61956)combinedwithaproprietarymixofpeptidesthatincludeCQIRFRFDGQPINE.TheantibodyhasbeenshowntoimmunoprecipitateawiderangeofSUMO-2/3targetedproteinsinaHeLacelllysate(Fig.1A).AlinearepitopehasnotbeenidentifiedandASM24appearstorecognizeaconformationalepitope.EachLotofantibodyisqualitycontrolledtoprovideahighbatchtobatchconsistency.TheLotspecificµgpertubecanbefoundintheLotspecificCOAdocumentsatwww.Cytoskeleton.com.ASM24ispurifiedbyProteinGaffinitychromatographyandissuppliedasalyophilizedwhitepowder.

EachLotofantibodyisqualitycontrolledtoprovideahighbatchtobatchconsistency.TheLotspecificµgpertubecanbefoundintheLotspecificCOAdocuments.


ValidatedApplications

Figure1:ImmunoprecipitationusingSUMO-2/3Antibody

DenaturedcelllysateswerepreparedfromHS43,CT37andKDS210(HS43:HeatShocktreated(43°Cfor10min),CT37:untreatedandKDS2:shRNASUMO-2knockdown).1mgoflysatewasusedfortheimmunoprecipitationofSUMO-2/3conjugates.IPexperimentswereperformedbytheprotocolpresentedinIPandWBMethod.Westernblotsofimmunopre-cipitatedproteinsweredevelopedusinganti-SUMO-2/3(Cytoskeleoncat#ASM23)(A)oranti-TFII-Iantibody(B).(A)Star(*)andcircle(o)indicateheavyandlightchainsofantibodies.Un-conjugatedfreeSUMOisdenotedbyatriangle.(B)UnconjugatedTFII-IisvisIBLenear120kDa.MultiplebandsindicatethatTFII-IisSUMOylatedbyseveralSUMO-2/3proteins.TFII-IhaspreviouslybeenreportedtobeatargetforSumoylation10,11.ToseethefullImmunoprecipitationprotocol,seetheproductdatasheet.

Anti-SUMO-2/3 IP
Figure2:ImmunofluorescenceofHeLacellsinmetaphasewithSUMO-2/3Antibody

HeLacellswerestainedandvisualizedbywidefieldfluorescencemicroscopyasde-scribedintheIFmethodbelow.Thecellswerestainedagainstα/β-tubulin(sheepanti-tubulinAb,Cat#ATN02,green)andSUMO-2/3(11G2,red).DNAwasstainedwithDAPI.MitoticcellsinmetaphasewereimagedwithaZeissAxioObserver.Z1microscope(1.4NA63Xobjective).ThelocalizationofSUMO2/3-conjugatesatchromosomescanbeobservedduringmitosisashasbeenpreviouslyreported12.ToseethefullImmunofluorescenceprotocol,seetheproductdatasheet.

Anti-SUMO-2/3 IF interphase

Figure3:ImmunofluorescenceofHeLacellsininterphasewithSUMO-2/3Antibody

HeLacellswerestainedandvisualizedbywidefieldfluorescencemicroscopyasde-scribedintheIFmethodbelow.Thecellswerestainedagainstβ-tubulin(sheepanti-tubulinAb,Cat#ATN02,green)andSUMO-2/3(11G2,red).DNAwasstainedwithDAPI.CellsininterphasewereimagedwithaZeissAxioObserver.Z1microscope(1.4NA63Xobjective).PMLnuclearbodies(nucleardots)werevisibleinSUMO-2/3stainingashasbeenpreviouslyreported5.ToseethefullImmunofluorescenceprotocol,seetheproductdatasheet.

Anti-SUMO-2/3 IF interphase

    Formoreinformationcontact:signalseeker@cytoskeleton.com

    AssociatedProducts:

    Signal-Seeker™SUMOylation2/3DetectionKit(Cat.#BK162)

    Signal-Seeker™SUMOylation2/3AffinityBeads(Cat.#ASM24-beads)

    Signal-Seeker™:BlastR™RapidLysatePrepKit(Cat.#BLR01)

    品牌介绍

    Cytoskeleton公司成立于1993年,专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、细胞骨架研究相关的系列试剂盒和产品,尤其以细胞骨架相关研究见长,既能满足于样品较少的科学研究,也可以用于小规模筛选研究和高通量大规模筛选研究。此外,公司还提供微管蛋白,肌动蛋白,小G蛋白,GAPs,GEFs等现有产品的药物筛选服务。

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