Product Uses Include
- Identification and characterization of cardiac actin binding proteins
- In vitro cardiac actin polymerization studies
Material:Bovine cardiac muscle actin is supplied as a lyophilized powder. When one vial is reconstituted in 1 ml of Actin Monomer Buffer: 5.0 mM Tris-HCI pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP and 0.5 mM DTT, it is at a final concentration of 1.0 mg/ml.
Purity:Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are > 99% pure.
Biological Activity:The biological activity is determined in three ways. The first method utilizes pyrene muscle actin as a fluorescent indicator of polymerization (use a 1:10 ratio of AP07 to AD99) . The second involves sedimentation of actin polymer by high speed centrifugation. The third method measures the integrity of the monomer by the specific inhibition of DNase activity. The biological activity is > 90%.
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
For a guide to performing actin polymerizations with this actin product please click here.
Sandberg, Alexander et al. “Fibrillation and molecular characteristics are coherent with clinical and pathological features of 4-repeat tauopathy caused by MAPT variant G273R.” Neurobiology of disease, vol. 146 105079. 19 Sep. 2020, doi:10.1016/j.nbd.2020.105079
Seervai, Riyad N H et al. “The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.” Science advances vol. 6,40 eabb7854. 2 Oct. 2020, doi:10.1126/sciadv.abb7854
Yang, Kai-Chien et al. “Deficiency of nuclear receptor interaction protein leads to cardiomyopathy by disrupting sarcomere structure and mitochondrial respiration.” Journal of molecular and cellular cardiology vol. 137 (2019): 9-24. doi:10.1016/j.yjmcc.2019.09.009
Mahmud, Z. et al. Structure and proteolytic susceptibility of the inhibitory C-terminal tail of cardiac troponin I. Biochim. Biophys. Acta - Gen. Subj. 1863, 661–671 (2019).
Butler S.C., et al. 2012. Inhibitory effects of pectenotoxins from marine algae on the polymerization of various actin isoforms. Toxicol. In Vitro. 26, 493-499.
Fressinaud C., et al. 2012. Axon cytoskeleton proteins specifically modulate oligodendrocyte growth and differentiation in vitro. Neurochem. Int. 60, 78-90.
Orlova A., et al. 2011. The N-terminal domains of myosin binding protein C can bind polymorphically to F-actin. J. Mol. Cell. 412, 379–386.
Debold E.P., et al. 2010. Human actin mutations associated with hypertrophic and dilated cardiomyopathies demonstrate distinct thin filament regulatory properties in vitro. J. Mol. Cell. Cardiol. 48, 286-292.
Passarelli C., et al. 2010. Susceptibility of isolated myofibrils to in vitro glutathionylation: Potential eelevance to muscle functions. Cytoskeleton. 67, 81–89.
Question 1: What is the isotype composition of the cardiac actin from bovine heart muscle?
Answer 1: Bovine cardiac actin (Cat. # AD99) is composed of 84% a-cardiac and 16% a-skeletal actin isoforms.
Question 2: How do I measure actin polymerization with the pyrene assay when there is no available pyrene conjugate for cardiac actin?
Answer 2: To examine the polymerization of unlabeled cardiac actin (Cat. # AD99), please click here for a polymerization protocol that uses an excess of the unlabeled [cardiac] actin plus 10% of pyrene-labeled skeletal muscle actin. The pyrene muscle actin will not polymerize on its own at the concentration used in this assay, so the reaction is dependent on unlabeled cardiac actin (Cat. # AD99) for F-actin formation. In this way, the pyrene-labeled muscle actin is taken up and polymerized to serve as a reporter for polymerization of the unlabeled actin that is present at a much greater concentration.
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。
本网站将在规定时间内给予删除等相关处理。
