Product Uses Include
- Discover and characterize α-actinin binding proteins
- Positive control of actin binding assays
- Positive control for actin bundling assays
Materialα-actinin has been purified from rabbit skeletal muscle. The protein is supplied as a lyophilized powder and upon reconstitution, will be in the following buffer: 4 mM Tris-HCl pH 7.6, 4 mM NaCl, 20 μM EDTA, 1% (w/v) sucrose, and 0.2% (w/v) dextran. The lyophilized product is stable at -70°C for at least 2 years. AT01 should be resuspended in nanopure water containing 1 mM β-mercaptoethanol or DTT to the desired working concentration.
PurityPurity is determined by scanning densitometry of the protein on an SDS-PAGE gel. α-actinin is >90% pure.
Figure 1: α-actinin protein purity determination. 20 µg of AT01 was run on an SDS-PAGE gel and stained with coomassie blue.
Biological ActivityAt 2.5 µM (0.25 mg/ml) α-actinin and 25 µM (1.0 mg/ml) F-actin at pH 7.0 and 24°C, approximately 70% of the α-actinin will co-sediment with F-actin after centrifugation at 150,000 x g for 1 h.
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
van der Gucht, J., Paluch, E., Plastino, J. and Sykes, C. (2005). Stress release drives symmetry breaking for actin-based movement. Proc. Natl. Acad. Sci. U. S. A. 102, 7847-7852.Lu, S., Carroll, S. L., Herrera, A. H., Ozanne, B. and Horowits, R. (2003). New N-RAP-binding partners α-actinin, filamin and Krp1 detected by yeast two-hybrid screening: implications for myofibril assembly. J. Cell Sci. 116, 2169-2178.Maul, R. S., Song, Y., Amann, K. J., Gerbin, S. C., Pollard, T. D. and Chang, D. D. (2003). EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 160, 399-407.Karakesisoglou, I., Yang, Y. and Fuchs, E. (2000). An epidermal plakin that integrates actin and microtubule networks at cellular junctions. J. Cell Biol. 149, 195-208.Waterman-Storer, C., Duey, D. Y., Weber, K. L., Keech, J., Cheney, R. E., Salmon, E. D. and Bement, W. M. (2000). Microtubules remodel actomyosin networks in Xenopus egg extracts via two mechanisms of F-actin transport. J. Cell Biol. 150, 361-376.
Question 1: What is the source of the alpha-actinin Cytoskeleton sells?
Answer 1: Cytoskeleton’s alpha-actinin (Cat. # AT01) is purified from rabbit skeletal muscle and has a purity of >85%.
Question 2: Can this alpha-actinin be used for actin bundling experiments?
Answer 2: Yes, Cytoskeleton’s alpha-actinin (Cat. # AT01) is well-suited for actin bundling experiments using skeletal muscle actin, it will not work with non-muscle isoforms of actin. Briefly, α-actinin is tested for biological activity by a co-sedimentation assay with Factin. At 2.5 μM skeletal muscle α-actinin and 25 μM actin in the form of F-actin, >70% α-actinin will co-sediment with F-actin. The reaction is carried out at pH 7.0 for 30 minutes at 24°C (at pH 8.0 α-actinin will not bind). Spin the mixture at 14,000 x g for 10 minutes. Probe a western blot with antibodies against alpha-actinin and actin. An SDS-PAGE gel is loaded with supernatant and pellet samples from alpha-actinin + actin and actin alone. After running the gel to separate samples it is stained with coomassie blue and then analyzed for band intensity at 43 and 116 kDal.
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。
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