Product Uses Include
- Inhibition of a wide range of proteases.
- Popular Activation Assay Component
MaterialProtease Inhibitor Cocktail is supplied as a white lyophilized powder. The cocktail contains the following protease inhibitors, Pepstatin A, Leupeptin, Benzamidine, Na-p-tosyl-L-arginine methyl ester (TAME). At 1X final working concentration the inhibitors are at 1 µM, 1.5 µM, 1 mM and 0.4 mM respectively.
Mode of Actionpepstatin A Reversibly inhibits some aspartic proteasesLeupeptin Reversibly inhibits trypsin like serine and some cysteine proteasesBenzamidine Inhibits serine proteasesTAME Inhibits arginine proteases, a thrombin and trypsin inhibitor
Storage and ReconstitutionLyophilized Protease Inhibitor Cocktail is stable at 4°C desiccated (<10% humidity) for 1 year.Each tube of Protease Inhibitor Cocktail should be resuspended in 1ml of DMSO (dimethyl sulfoxide) to give a 100X strength stock. The reconstituted stock can be stored at 4°C for up to 6 months or at -70°C for up to 1 year.
Related ProductsThis protease inhibitor cocktail is used in the following Cytoskeleton Activation Assays:
- Ras activation assay Biochem kit (Cat. # BK008)
- Cdc42 activation assay Biochem kit (Cat. # BK034)
- Rac activation assay Biochem kit (Cat. # BK035)
- RhoA activation assay Biochem kit (Cat. # BK036)
- G-actin : F-actin in vivo assay Biochem kit (Cat. # BK037)
- Microtubule : tubulin in vivo assay Biochem kit (Cat. # BK038)
- RhoA G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK121)
- RhoA G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK124)
- Rac1,2,3 G-LISA™ Activation Assay Kit (colorimetric) (Cat. # BK125)
- Rac1 G-LISA™ Activation Assay Kit (luminescence) (Cat. # BK126)
- Cdc42 G-LISA™ Activation Assay (Cat. # BK127)
- Rac1 G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK128)
- RalA G-LISA™ Activation Assay (Colorimetric Based) (Cat. # BK129)
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
V. Munnamalai et al. 2014. Bidirectional interactions between NOX2-type NADPH oxidase and the F-actin cytoskeleton in neuronal growth cones. J. Neurochem. doi: 10.1111/jnc.12734.
H. Vindin et al. 2013. Validation of an algorithm to quantify changes in actin cytoskeletal organization. J. Biomol. Screen. 19, 354-68.
D. Chand et al. 2012. C-terminal region of teneurin-1 co-localizes with dystroglycan and modulates cytoskeletal organization through an extracellular signal-regulated kinase-dependent stathmin- and filamin A-mediated mechanism in hippocampal cells. Neurosci. 219, 255–270.
Coming soon! If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。
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