Product uses include
- Screening for cancer tubulin specific ligands.
- Using SPA technology (Amersham) for drug screening.
- Microinjection and tubulin distribution in living cells.
- Immobilizing tubulin onto a solid surface via streptavidin.
MaterialHeLa cell tubulin (Cat. # H001) has been modified so that random surface lysines contain a covalently linked, long-chain biotin derivative. A long-chain biotin derivative was selected for this procedure because it allows the biotin molecules to be spaced far enough away from the tubulin protein so as not to interfere with its activity, e.g., ligand binding to SPA and other streptavidin based reagents. Biotin labeled tubulin is supplied as a lyophilized powder.
PurityThe HeLa cell tubulin is >90% pure (see Cat. # H001). Labeling stoichiometry was determined to be approximately one biotin per tubulin heterodimer.
Biological ActivityBiotin HeLa cell tubulin has polymerization properties that are indistinguishable from those of unlabeled tubulin.
For product Datasheets and MSDSs please click on the PDF links below. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice@cytoskeleton.com
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Question 1: Can biotin-labeled HeLa tubulin polymerize just as well as unlabeled HeLa tubulin?
Answer 1: Yes, the biotin-labeled HeLa cell tubulin (Cat. # H003) polymerizes in a similar manner to unlabeled HeLa cell tubulin (Cat. # H001). In QC testing, the biological activity (polymerization) of biotin HeLa tubulin has been tested as follows: 250 ug of biotin HeLa tubulin are reconstituted to 2 mg/ml in G-PEM buffer pH 6.9 plus 20% v/v glycerol and incubated at 35°C in a cuvette. The optical density at 340 nm is taken at time intervals. A value of 0.075 OD unit indicates that >90% of the tubulin has polymerized.
Question 2: What are some specific uses for biotin-labeled HeLa tubulin?
Answer 2: Cytoskeleton’s biotin-labeled HeLa cell tubulin (Cat. # H003) can be used 1) to screen for cancer tubulin specific ligands, 2) for microinjections into living cells to track tubulin distribution, 3) immobilizing tubulin onto a solid surface via streptavidin, and 4) with SPA technology (Amersham, Inc.) for drug screening.
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、细胞骨架研究相关的系列试剂盒和产品，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有产品的药物筛选服务。
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